EVALUATION OF THE EMBRYOTOXIC EFFECT OF LECTIN FROM Bothrops leucurus (BlL) IN THE Gallus gallus domesticus MODEL
DOI:
https://doi.org/10.16891/2317-434X.v12.e1.a2024.pp4077-4087Abstract
A type c lectin isolated from the venom of the Bothrops leucurus snake showed important effects such as antibacterial, antitumor and leishmanicidal activity against L. braziliensis and L. amazonensis. To analyze the possible embryotoxic effects of Bothrops leucurus Lectin (BlL), an experimental model was chosen with Gallus gallus domesticus embryos. In this research, 180 fertilized eggs of G. gallus domesticus were used, due to the similarities of the initial stages in the embryonic development of birds and humans, in addition to the characteristics and advantages that benefit their use for toxicity testing. The concentrations applied to the embryos were 2.5 and 5 μM BlL, using the Total Mounting, Transverse Section and HET-CAM techniques. After carrying out the procedures, the embryos were analyzed using optical microscopy. Concluding that, regardless of the concentrations tested during the study, BlL did not cause irritability in the chorio-allantoic membrane and did not alter the morphogenesis of the central nervous system in G. gallus domesticus embryos.