EFFECT OF SULFORAPHANE ON IN VITRO PRODUCTION OF BOVINE EMBRYOS
DOI:
https://doi.org/10.16891/2317-434X.v13.e3.a2025.id2739Keywords:
Antioxidant, Biotechnology, Oxidative stressAbstract
The aim of this study was to evaluate the antioxidant effects of sulforaphane (SFN) on bovine embryo production in vitro. Ovaries from slaughterhouses were collected to obtain viable oocytes, which were selected and matured for 24 h in an incubator under a controlled atmosphere. Mature oocytes were fertilized and incubated for 9 h. After this period, 5 µM SFN was added in the final third of the in vitro fertilization step (final 3 h). Then, the probable zygotes were cultured, and embryonic development was evaluated at the cleavage, morula, blastocyst, and hatching stages. The data obtained were analyzed using the Wilcoxon test at a 5% significance level. No significant difference was observed in the cleavage rates (79.07% vs. 78.06%) and morulae (61.36% vs. 54.66%) between the Control and SFN groups. In contrast, the blastocyst rate (34.53% vs. 27.24%) and hatching rate (22.0% vs. 15.36%) were significantly higher in the Control group than in the SFN group. The addition of SFN in the final stage of in vitro fertilization did not significantly improve embryo production rates for bovine species. Further studies are needed to elucidate the effects of this antioxidant in in vitro culture systems of embryonic cells.