PROSPECTING FUNGI OF INDUSTRIAL INTEREST ISOLATED FROM SOIL IMPACTED WITH SOLID HEALTH WASTE

Abstract

The soil composition of landfills presents a diversified microbiota, which acts in the decomposition and biorecovery of the soil, in addition to producing essential extracellular compounds and enzymes with bioactivity of industrial and biotechnological interest. Based on this, the present project aims to evaluate the enzymatic activity of microorganisms isolated from the soil of dumps in the region of Apicum-Açu, Maranhão, Brazil. For isolation of microorganisms, soil samples were subjected to serial decimal dilutions and inoculated in conventional culture medium, at 28ºC for 7 days, for isolation and numbering. The microculture technique was used to analyze the morphological characteristics and identify the isolated microorganisms at the genus level. Subsequently, for qualitative enzymatic analysis, protease and lipase tests were performed, in specific culture medium, with incubation at 28ºC under constant observation over a period of 72 hours to 7 days. Of the three isolates from the soil of a landfill in the city of Apicum-Açu, it was possible to identify macro and microscopically the presence of and Aspergillus sp. All isolates were positive for protease production, while only isolates 2 and 3 were positive for lipase production. Thus, the importance of the search for microorganisms of interest present in different biomes and environmental conditions, such as the landfill soil worked on in the study, is evident, thus requiring more in-depth studies of these isolates, which will allow a more complete characterization of its benefits for the technological and environmental industry.