EVALUATION OF THE ANTIBACTERIAL AND MODULATORY ACTIVITY OF EUGENOL

Abstract

Bacterial resistance is a major problem in the treatment of infections, especially nosocomial infections. The production of new antimicrobial agents is dwindling, so studies with medicinal plants and compounds obtained from them are necessary for the identification of biologically active substances, such as Eugenol. The objective of this researhc was to evaluate the bacterial and modulatory reaction capacity of the Eugenol molecule against the concentration and multiresistant patterns of Escherichia coli and Staphylococcus aureus. The antibacterial and modulatory activity was tested as a bacterial line using disc diffusion, through a 0.5-fold inoculum of the MacFarland scale in BHI broth, which was subsequently sown in Müeller-Hinton, added with discs of Chloramphenicol and Sulfonamide isolated and combined with Eugenol and incubated in an oven at 37ºC, with subsequent measurement of the inibition halos. Eugenol showed bacterial inibition results at no diluiton product in all specimens tested and associated with Chloramphenicol, it showed a synergistic effect against Escherichia coli 25922, antagonistic effect against E. coli 27 and in both strains of Staphylococcus aureus there was an antagonistic relationship, and with Sulfonamide resulted in antagonism in the two strains of S. aureus and in strain E. coli 27, while in strain 25922 there was a statically non-significant increase in the stretch halo. In view of the results, it was highlighted that Eugenol is an antibacterial agent, in addition to interfering in some way in the action of drugs already marketed, so there is a need to study the interaction of this substance with other antimicrobials as an alternative in the fight against microbial resistance.